Identification of a major surface protein on Neospora caninum tachyzoites
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چکیده
منابع مشابه
Susceptibility of various cell lines to Neospora caninum tachyzoites cultivation
Neospora caninum is a coccidian protozoan parasite which is a major cause of bovine abortions and neonatal mortality in cattle, sheep, goat and horse. Occasionally, cultured cells are used for isolation and multiplication of the agent in vitro with several purposes. In this study the tachyzoite yields of N. caninum were compared in various cell cultures as the host cell lines. Among the cell cu...
متن کاملsusceptibility of various cell lines to neospora caninum tachyzoites cultivation
neospora caninum is a coccidian protozoan parasite which is a major cause of bovine abortions and neonatal mortality in cattle, sheep, goat and horse. occasionally, cultured cells are used for isolation and multiplication of the agent in vitro with several purposes. in this study the tachyzoite yields of n. caninum were compared in various cell cultures as the host cell lines. among the cell cu...
متن کاملIntrauterine Neospora caninum inoculation of heifers and cows using contaminated semen with different numbers of tachyzoites.
OBJECTIVE To investigate the potential of different Neospora caninum tachyzoite doses to infect heifers (experiment 1) and cows (experiment 2) when administered in utero by artificial insemination via contaminated semen. METHODS In experiment 1, five groups of 5, 7, 8, 9, and 5 cyclic heifers were hormonally synchronized and artificially inseminated with semen containing 0 (A, controls), 10(2...
متن کاملIn vitro effects of novel ruthenium complexes in Neospora caninum and Toxoplasma gondii tachyzoites.
Upon the screening of 16 antiproliferative compounds against Toxoplasma gondii and Neospora caninum, two hydrolytically stable ruthenium complexes (compounds 16 and 18) exhibited 50% inhibitory concentrations of 18.7 and 41.1 nM (T. gondii) and 6.7 and 11.3 nM (N. caninum). To achieve parasiticidal activity with compound 16, long-term treatment (22 to 27 days at 80 to 160 nM) was required. Tran...
متن کاملCharacterization of Neospora caninum surface protein NcSRS2 based on baculovirus expression system and its application for serodiagnosis of Neospora infection.
The baculovirus expression system has proved to be a useful tool for the production of recombinant proteins. Here we have characterized the Neospora caninum surface protein NcSRS2 produced by two types of the recombinant virus and also have developed an enzyme-linked immunosorbent assay (ELISA) using recombinant NcSRS2 for the serologic diagnosis of Neospora infection. Western blot analysis sho...
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ژورنال
عنوان ژورنال: Parasitology Research
سال: 1996
ISSN: 0932-0113,1432-1955
DOI: 10.1007/s004360050152